PerkinElmer

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⮱Drug discovery is a complex, costly, and time-consuming undertaking. And for research scientists, high-throughput screening is where the journey to discovery begins.

⮱Your job is to move from target to hit to candidate quickly and efficiently – with as little wasted resources as possible. Our HTS screening reagents do just that, providing you with a wide range of validated reagents and kits in Alpha, LANCE®, DELFIA®, and other assay technologies.

⮱Accelerate your workflow, while getting consistent, accurate, and physiologically relevant results.

AlphaLISA assay principle
AlphaLISA assay principle

Alpha Technology

⮱Virtually anything can be measured with Alpha Technology quickly and easily, including enzymes, receptor-ligand interactions, low-affinity interactions, second messenger levels, DNA, RNA, proteins, protein post-translational modifications, protein:protein interactions, peptides, sugars and small molecules.

  • Ease-of-use: homogeneous, no-wash assay, with no separation steps
  • Fast: results in 3 hours for most applications
  • High sensitivity: detect down to femtogram levels of target
  • Large dynamic range: 4-5 logs
  • Compatible with many samples types: serum, plasma, cerebrospinal fluid (CSF), tissue extracts, cell culture supernatants, cell lysates, and more

⓪Choose the Alpha solution that’s right for you⓪

  • AlphaLISA® – when running large numbers of ELISA from the same sample or working with difficult matrix, serum or plasma
  • AlphaLISA SureFire® Ultra and AlphaScreen® SureFire – an excellent choice for studying pathways or phosphorylated proteins in whole cells, or studying endogenous receptors
  • AlphaPlex – for measuring more than one analyte from the same well, by using the specific wavelengths of Europium, Terbium and Samarium atoms.
  • Alpha SureFire Ultra Multiplex – for measuring two phospho-events or one phospho and a “total” protein control from the same well.
  • AlphaScreen® – perfect for studying fusion-tagged proteins or protein: protein interactions, and for testing cAMP, phosphorylated peptides or samples in a simple matrix. Available as AlphaScreen, AlphaLISA and AlphaPlex products.
  • Alpha Toolbox – when your target doesn’t have an off-the-shelf kit, the toolbox gives you everything you need to create your own. Available as AlphaScreen, AlphaLISA and AlphaPlex products.
  • Alpha Biomarker kits – more than 200 validated and ready to use sandwich immunoassays. Available as AlphaLISA and AlphaPlex products.
LANCE biomarker detection assay principle
LANCE biomarker detection assay principle

LANCE Ultra TR-FRET检测

LANCE Ultra技术可为研究人员提供从试验立项到初次和二次筛选の高质量操作简单の试验,。满足多模式探测器各类靶标の试验要求,。市场上可购买TR-FRET试验,。试验时间短,。
  • 免洗涤/无分离步骤
  • 均相分配开盒即用技术
  • 灵敏检测
  • 重现性高
  • 测定时间短
  • 便于自动化
  • 384孔板和1536孔板
LANCE和LANCE(镧系元素螯合物激发)Ultra是TR-FRET(时间分辨荧光共振能量转移)和均相(无需洗涤)技术,。其中一个结合配体标有供体荧光团(LANCE铕螯合物),。另一个标有受体荧光团(ULight™受体染料),。激发到320-340nm时,。如果距离范围满足荧光共振能量转移要求(~10 nm),。则能量可从供体螯合物转移到受体荧光团上,。因此可在665nm处探测到光线发射,。
DELFIA_trf
DELFIA_trf

DELFIA

⮱DELFIA (Dissociation-Enhanced Lanthanide Fluorescence Immunoassay) is a proven, robust immunodetection platform based on time-resolved fluorescence (TRF) detection. This detection method, coupled with the unique chemical properties of lanthanide chelates, makes DELFIA a superior alternative to conventional ELISA for research, drug discovery, and clinical applications.

⮱DELFIA offers a combination of high sensitivity, wide dynamic range and long-lasting stability that traditional ELISA cannot match. 

Reporter gene assay
Reporter gene assay

Reporter Gene Assays

Whether your assay requires the sensitivity provided by high signal intensity, or the flexibility of an extended signal half-life, PerkinElmer provides a solution. Choose from one of three luciferase detection systems: neolite, britelite plus, or steadylite plus depending on the specific requirements of your experiment.
  • ⓪ No more washing⓪ – just add your lites reagent of choice, mix, incubate, and read
  • ⓪Hassle-free storage⓪ – all lites reagents are designed for extended storage at 2-8 °C (no -20 °C storage required)
  • ⓪Odor-friendly⓪, eliminates hood work – lites products do not contain DTT and do not have the hazards and repugnant odor typical of conventional luciferase detection systems
  • ⓪Excellent Z’ values⓪ – superior S/B ratios allow for excellent Z’ values in antagonist and agonist studies
ATPlite assay principle
ATPlite assay principle

ATP Quantitation

⮱For fast, reliable detection of ATP for cell quantitation, ATPlite 1step and ATPlite are innovative technologies that measure cell proliferation and cytotoxicity in mammalian cells based on the detection of ATP using firefly luciferase.

⓪ATPlite 1step – Highest sensitivity⓪

  • True “mix and measure” continuous processing
  • Ultra high sensitivity, 3 times more light output than ATPlite
  • Short equilibrium time for faster processing
  • Signal half-life of 30 minutes

⓪ATPlite – Convenient, extended Signal⓪

  • 2-step format, non-separation assay for time course studies; lysate can be stored frozen and tested later
  • Quantitate from <5 cells/well
  • Prolonged equilibrium time for batch processing
  • Signal half-life of 5 hours
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